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**Methods:** All patients with node-positive breast cancer treated with trastuzumab and neoadjuvant chemotherapy at Stanford between 2001-2018 were analyzed. Patients were classified into no PMRT, low dose PMRT (LD-PMRT) (\50Gy). Disease-free survival (DFS) was examined using Kaplan-Meier, and differences between groups were calculated using a log rank test. A Cox regression analysis was used to determine the impact of PMRT on recurrence-free survival (RFS) using trastuzumab and PMRT as factors, as well as the impact of PMRT on margin positivity and nodal disease. Multivariable models were adjusted for time-dependent covariates. All analyses were two-sided.

**Results:** A total of 489 patients were included in the study, 194 in the no PMRT group, 103 in the LD-PMRT group, and 192 in the HD-PMRT group. Median follow up was 5.6 years. A significantly lower rate of local recurrence (HR 0.34, CI=0.19-0.59, p=0.0001) was noted in the HD-PMRT group compared to the no PMRT group. A significantly lower rate of margin positivity (HR 0.62, CI=0.41-0.94, p=0.023) was noted in the LD-PMRT group compared to the no PMRT group. However, the rate of margin positivity in the HD-PMRT group was not significantly

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A method to simultaneously monitor the generation of all known psoralen photoproducts in human and animal skin was developed. The reaction was initiated by using psoralen-irradiated skin that was immersed in isotonic saline. At the completion of the reaction, the skin surface was removed and the epidermal layer was separated from the dermal layer by tape-stripping. The skin surface and the epidermal layer were subsequently extracted with toluene/isopropanol and the extracts were evaporated to dryness. The extracts were reconstituted in methanol and analyzed by LC-tandem mass spectrometry (MS/MS). The different psoralen photoproducts were identified using appropriate reference standards and fragmentation data. With the exception of 6,8-methoxypsoralen and 5-methoxypsoralen, all other psoralen photoproducts generated by irradiation of human skin with a 1.0 J cm-2 dose of UVA or UVB could be identified. The 5-methoxypsoralen was identified after irradiation of human skin using UVA

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