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We have isolated and characterized the human glutathione peroxidase 1 (GPX-1) gene promoter. By bioinformatic analysis of the genomic sequence, a human GPX-1 gene (hGPX-1) was identified as one of three possible genes encoding glutathione peroxidase in a human genomic library. There were two copies of hGPX-1 gene in human genomic library. A structural gene of hGPX-1 gene was 15 kb on average, and was dispersed into approximately 5 kb of coding sequence on the complementary strand. Human hGPX-1 gene was located at q33.1 band of human chromosome 12. To elucidate the mechanism for the enhanced expression of hGPX-1 in human hepatocellular carcinoma, we have functionally characterized the promoter region of the gene. A series of 5′-end deletion mutants of hGPX-1 promoter, including hGPX-1–578, hGPX-1–450, hGPX-1–200 and hGPX-1–100, were fused to the luciferase reporter gene, and the transcription of reporter gene was investigated by transient expression assay in human HepG2 cells. Using the deletion mutants of the promoter region, it was found that a 68-bp fragment from -100 to -28 was important for high transcriptional activity. Deletion of the fragment from -100 to -49 or -48 to -28 completely abrogated luciferase expression,
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