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An RNAi screen to identify genes involved in megakaryocytopoiesis and erythropoiesis.
In this study, we describe the design and performance of a high-throughput RNAi-based screen to identify genes important for megakaryocytopoiesis and erythropoiesis. Our goal was to identify genes that mediate feedback regulation between megakaryopoiesis and erythropoiesis by knocking down key genes and testing the impact on megakaryocytopoiesis and erythropoiesis. Specifically, we first generated megakaryocytic cell lines from murine bone marrow. Using this cell model, we screened a library of 19,092 shRNAs in an RNAi knockdown screen. From this screen, we selected a set of validated target genes, including AKT1, BMX, FGR, MLLT3, and UBE2V1, which have not been previously linked to megakaryopoiesis or erythropoiesis. We then interrogated these genes for their impact on megakaryopoiesis and erythropoiesis in vitro. We identified several genes with a robust impact on megakaryocytopoiesis and erythropoiesis in vitro, including AKT1, FGR, FGR3, KLF1, and TGFBR2. In addition, we uncovered several genes previously shown to be involved in megakaryopoiesis and erythropoiesis; however, no previous study had evaluated these genes in the context of megakaryopoiesis or erythropoiesis. In summary, we have used a high-throughput RNAi-based screen to identify novel genes that regulate megakaryocytopoiesis and erythropoiesis.Q:
Ignore row in loop in datagridview
I’ve added some error in the rows of the datagridview. So I need to ignore the row with the error.
I’ve tried this code:
for (int i = dgvR.Rows.Count – 1; i >= 0; i–)
{
if (dgvR.Rows[i].Cells[“VATNumber”
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