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To study the association between TP53 expression and radioresistance of HepG2 cells. The adenovirus-mediated TP53 gene transfer model was established in HepG2 cells. The radiation sensitivity of HepG2 cells was measured by single-dose and multi-dose sensitivities. The cell apoptosis rate was detected by flow cytometry. The activation of p53 and p21 was detected by Western blot. The protein expression of thymidylate synthase (TS) was measured by Western blot. Data were analyzed by SPSS10.0 software. TP53 overexpression significantly elevated radiation sensitivity of HepG2 cells. It significantly increased cell apoptosis rate of HepG2 cells and p53 expression. Overexpression of TP53 reduced the protein expression of TS, which also contributed to increased radiosensitivity of HepG2 cells. Overexpression of TP53 can enhance radiosensitivity of HepG2 cells by inducing cell apoptosis and reducing protein expression of TS.The Young Women’s Choir
The Young Women’s Choir was an English vocal ensemble founded in 1854 by Harry Gill and Charles Burney. It was open to girls of the “choir-school” type, although some of the girls belonged to the “divinity-school” type. It was popular and often appeared at concerts. The Young Women’s Choir had a significant effect on the London style of English choral singing.
Founding
Founded in 1854, the choir was formed by Harry Gill and Charles Burney, and their youngest brother Herbert Burney. Herbert left the choir in 1855, and the other three continued until 1858. Herbert was replaced by Cecil Pinsent, who conducted his first concert with the choir in September 1858.
During its existence the choir frequently appeared at the
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Descargar Cdma Workshop 335 Crack
Descargar Cdma Workshop 335 Crack
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{
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