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You need to re-run the installer. There’s a small chance that you already have a full version installed on your machine.
Also, make sure you only have one instance of the autocad install on your machine and make sure you are using the 32bit version of autocad 2009 and not the 64bit version.
After 3 or 4 weeks of feeding, SBS is not a big problem in most cases. The LPO should be fine for it.
Sanitize the beakers between experiments with 10% H~2~O~2~. H~2~O~2~ is an oxidizer, so it is a good sanitizer (often the safer way of neutralizing any acidity). The LPO will be a bit fragile when activated, so do this after all your liquid has been aspirated, though much of it will evaporate and the LPO will be fine even a day after activation.
Re-pair your filter manifold with the LPO. I would not waste your HCl on the filter manifold.
The LPO will have a discharge port on the back, use a syringe to aliquot into a beaker, then transfer to a fresh beaker. Again, do this right after activation of the LPO so that as little liquid evaporates. Be careful when injecting the LPO to make sure that no air bubbles get into the LPO.
If using the HDPE tubing, first fill a syringe with the LPO and then syringe into the tubing. Once a 10 gram sample has been taken, flush the tubing and disconnect the syringe and beaker. Rinse the tubing and beaker with deionized water, then begin doing the experiment again with the next liquid sample.
If a reaction failed, start over or do some troubleshooting, read the suggested troubleshooting section below.
I am using a 200 mg scale that is recommended by the company and I am doing a 40 minute oxidation process on 80 grams of tepid water. I have run one experiment and am on the second. I put the beaker containing the LPO inside a beaker containing the HCl. Is it ok that the beakers are touching one another? I want the LPO’s outlet to touch the HCl’s input but am afraid that the two will touch each other or the tubing as I pour the LPO into the HCl.
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Homology of $S^1$ minus 4 points on the circle
I’m trying to use Mayer-Vietoris to calculate $H_2(S^1 \setminus 4 \text{ points})$.
It is clear I will get something like $Z \oplus Z$, but what would I have to do to get it?
I also realize the answer should be the fundamental group of the complement, but I don’t think I can find it.
Thanks!
A:
This is a complete answer, as I think it was originally meant to be.
Mayer-Vietoris gives a homology isomorphism
$$H_n(X)\oplus H_n(X,X\setminus U)\to H_n(X\setminus U)$$
for a closed subspace $U$ and the space $X$ minus $U$ and $X\setminus U$. In our case, $U$ is the open circle minus 4 points $U\simeq\mathbb{D}^2\setminus\{p_1,\dots,p_4\}$ and $X$ minus $U$ is the circle minus the open disk $X\setminus U\simeq\mathbb{S}^1\setminus\mathbb{D}^2\simeq\mathbb{S}^1$.
Then the above results gives
$$H_n(\mathbb{S}^1\setminus\mathbb{D}^2)\simeq H_n(\mathbb{D}^2)\oplus H_n(\mathbb{D}^2\setminus\{p_1,\dots,p_4\})\simeq H_n(\mathbb{D}^2)\oplus \mathbb{Z}_2\oplus \mathbb{Z}_2\simeq \mathbb{Z}_