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The video is uploaded only to provide the download linkA human monoclonal antibody to human gamma-globin gene mRNA directs the synthesis of a precursor of gamma-globin protein in a human cell line.
We have produced a human monoclonal antibody (MAb) to gamma-globin mRNA by using the technique of immunoglobulin-gene transfection. The MAb 1D6, which has a heavy chain of IgG1 and light chain of kappa, was used to affinity-purify membrane fractions from a human erythroleukemia cell line (K562) and fluorescein isothiocyanate-labeled mRNA for affinity purification. The membrane fraction bound mRNA by a process thought to be specific because the MAb could not cross react with other mRNAs tested. In immuno-precipitation experiments, the membrane fraction bound to the protein products of the isolated gamma-globin mRNA. The MAb 1D6 also inhibited the synthesis of the protein product in a cell-free translation system using human gamma-globin mRNA in addition to the recombinant ribosome. The inhibition of the synthesis of the protein product in the cell-free system required the intact translation initiation signal of the mRNA. These data support the notion that the MAb 1D6 recognizes the translation initiation signal of human gamma-globin mRNA. In addition, the gene product of the mRNA might be synthesized as a precursor of a gamma-globin protein. These results are the first demonstration of the gene-specific translation of a naturally occurring mRNA in a eukaryotic translation system.
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Apple plans to open 3-million square feet of production in India. plans to open 3-million square feet of production in India, Reuters. New Delhi’s Capital is the one of the great cities of the world with many.. 6,000 square meters of retail and office space would also be built. In June, it’s next year that Apple is.
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In The
Eleventh Court of Appeals
__________
No. 11-11-00055-CR
__________
RICHARD RAY TAYLOR,
Appellant
V.