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The p38/MAPK (mitogen-activated protein kinase) family is activated by various stress-inducing agents, including NMDA and neurotrophins, and has been suggested to play a role in the N-methyl-D-aspartate receptor (NMDAR)-mediated neuronal death. We found that the activation of p38 by a superagonist, such as a high concentration of spermidine, high K+, but not Na+, induced a sustained increase in intracellular Ca2+ and an elevation of cytosolic free Ca2+ concentration in cultured hippocampal neurons by the stimulation of NMDARs. In addition, blocking the activation of p38 with a highly specific inhibitor attenuated the Ca
A: I could try to download the files, but if you are asking for a detailed explanation for this, I’d have to research what they are from the start. Clinical testing of a multi-layer biosensor film for detection of respiratory virus in nasopharyngeal aspirate. A multi-layer film using a temperature-controlled heat-stable water-based medium for sensitive and specific detection of respiratory viruses is a useful method for patients with respiratory disease and their caregivers. The characteristics of this multi-layer film for detection of respiratory viruses were investigated. We developed a multi-layer film based on immunochromatography (IC) method, where a test strip had two positive controls: bovine serum albumin-normal monoclonal antibody (MAb), and a positive control antigen (PCA)-PCR amplified RNA. A preservative solution containing 0.01 M PBS and 0.1 M sodium azide was added to the IC test strip. A multi-layer film was developed to detect respiratory viruses in nasopharyngeal aspirate (NPA), and the characteristics of the multi-layer film were investigated. (1) The respiratory viruses were extracted from NPA by guanidinium-thiocyanate/3-[(3-cholamidopropyl) dimethyl amino]-1-propanesulfonate (CHAPS)/NaCl and 0.01 M PBS/NaN(3). Then the RNA sample was purified by phenol/guanidine thiocyanate solution/EDTA (total volume, 10 ml) and extracted by a polypropylene-saturated phenol and isoamyl alcohol. The RNA samples were introduced into the multi-layer film and the mixed solution was prepared. The result of sensitivity and specificity was 100% (10/10). (2) Influenza A virus (10(-4) TCID50) mixed with culture medium, potato glycoprotein toxin, and cytochalasin B could not disturb the result. (3) The same clinical samples tested by IC and membrane hybridization assay showed positive results in 6 (40%) of 15 samples. This result suggests that the sensitivity of the IC test was similar to that of the membrane hybridization assay, and that IC could provide a convenient tool for the clinical diagnosis of respiratory diseases.— author: – | Lingjie Li^1^ Jiangtao 1cdb36666d
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