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As you get to know Photoshop, don’t become complacent. You need to be constantly upgrading your skills. Also, don’t forget that it’s not the only photo editing tool; GIMP (covered in the next chapter) and Photoshop Elements (covered in Chapter 19) are much cheaper and equally powerful. Figure 6-1A shows an example of the _Save for Web_ dialog box for a simple layer. **Figure 6-1A:** The Save for Web dialog box. Photoshop Elements automatically formats files with the best compression to reduce bandwidth utilization for the web and downloads faster. Photoshop Elements makes a task of resizing images for the web by converting all formats to JPEGs and attaching thumbnails to images. To create a good thumbnail, use JPEG quality setting 80. Always use the _Open_ or _Save_ button to save a file as its new file format or size. The original image (A) and the result of the resizing in Photoshop Elements (B) are shown in Figure 6-1B. Photoshop Elements also creates the largest thumbnails possible without ruining image quality. With the Layers dialog box open, choose the Info tool (or the Layer Information icon). Figure 6-1C shows the Layers dialog box. **Figure 6-1B:** Saving a file creates the new file in its new format and size. **Figure 6-1C:** You can get information about a layer using the Layers dialog box. You may have to adjust the pixels per inch (ppi) for a JPEG file in order for the final image to be a satisfactory size. Figure 6-1C shows an example of using the Layers dialog box to get information for a layer. Photoshop Elements makes it easy to hide or remove objects or layers from your canvas before saving your image. To hide a layer, simply click it and press the H key on your keyboard. To remove objects or layers from the canvas (or the file), use the Eraser tool and follow these steps: 1. Select the Eraser tool. 2. Press and hold down the Shift key and drag the Eraser tool to your canvas. 3. Release the Shift key and delete the objects or layers you want to remove. To see the canvas on which you’re working, choose View⇒Canvas Size
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There are many great reasons to start using Photoshop Elements instead of Photoshop. You can use Elements to edit photos with ease, even if you are just looking to crop an image, or if you want to print out an image. Photoshop Elements has far fewer settings than Photoshop, so it is more user friendly, especially if you are not an expert in the field. With Photoshop Elements, you can edit your images in three different ways: using the included wizards, or by using a toolbox and menus, and using either the standard Photoshop Elements or Full Photoshop interface. The main difference between the Photoshop Elements and Photoshop interface is that the Photoshop Elements interface uses toolbars and folders to organize different features. This can be confusing if you are an expert in Photoshop, but Elements is the perfect alternative to Photoshop because it has fewer options. If you are new to Elements and want to get started using it, we have created the following tutorial to help you: Learn how to use Photoshop Elements to edit photos Using Elements to edit photos is actually a lot easier than using Photoshop, as Elements has limited options. If you are a beginner, you should start out by using the simpler interface and begin using Photoshop Elements to edit images. If you are skilled in Photoshop and want to edit images using the full Photoshop interface, you can edit elements with the Elements tools and menus, like you would in Photoshop. 1. Selecting the photo you want to edit Open your photo in the program you want to edit (e.g. Photoshop Elements). You can either use the main Elements window or the Windows Photo Viewer. You can also import a photo from your computer by clicking on the Import button at the top right of the screen. 2. Using the crop tool If you want to edit an image using Photoshop Elements or Photoshop, you need to select the area of the photo you want to edit. The crop tool (the fourth from the left) is used to select an area on the photo. It is at the top right corner of the window. Press and hold the SHIFT button, then click and drag on the area on the photo you want to edit. To get a full overview of your photo, you can use the Zoom tool (the zoom bar at the bottom). 3. Cropping the photo When you are done cropping, press DELETE to remove the image from the active window. In 388ed7b0c7
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% BSA in blocking buffer for 1 h at RT. Primary antibodies used were: mouse monoclonal M2, 1:2000 (Chemicon International, Inc.); mouse monoclonal, 1:300 (Sigma-Aldrich Inc.). After washing three times with washing buffer, membranes were incubated with either anti-mouse or anti-rabbit secondary antibody conjugated to IR-fluorescence (1:2000; Molecular Probes, Eugene, Oregon, USA) for 1 h at RT, washed three times with washing buffer and imaged by Odyssey infrared scanner (LI-COR, Lincoln, Nebraska, USA). Additional information ====================== **How to cite this article:** Lunde, H. S. *et al*. CRK acts as a survival kinase for the *Drosophila* *Cdc42* effector OSR1. *Nat. Commun.* 6:6486 doi: 10.1038/ncomms7486 (2015). Supplementary Material {#S1} ======================
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