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Enter the name of your academic institution. Our email will be able to support you in case any questions should arise. Get started Now!In the present work, we made the first study of gene ontology (GO) of proteins encoded by a unique type of micro RNAs, microRNAs (miRNAs) of the Ovirinae subfamily (ssRNA). A total of 157 miRNAs were identified in the white-tailed deer (Odocoileus virginianus) genome. Their target genes are known to be involved in a number of biological processes, such as cell growth and maintenance, cell differentiation, and cell adhesion. In addition, miRNAs are also targeted to viral genes involved in transcription and translation. These miRNAs may be associated with the physiology of this species and the defense against pathogenic viruses.

**Significance & Innovation**

This work is the first report about the function of miRNAs in the deer family. We analyzed the sequences and secondary structures of miRNAs in the deer genome to develop an improved method for the identification of miRNAs. In addition, the target genes of miRNAs were identified and their enrichment for genes involved in biological processes were analyzed. The results show that miRNAs in the deer genome are highly involved in biological processes and have potential associations with the physiology and immune responses of deer.

**Supporting information**

Figure S1 = The secondary structures of deer mature miRNAs. Figure S2 = Target genes of miRNAs identified in the white-tailed deer genome. Table S1 = The features of the identified deer miRNAs. Table S2 = Target genes of miRNAs predicted by miRanda.Table S3 = Target genes of miRNAs predicted by PITA. Table S4 = The gene ontology of the predicted target genes of miRNAs of deer.
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