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It has been hypothesized that stimulation of bacterial translocation in the early stage of hepatic failure may contribute to the enhanced susceptibility of hepatic failure patients to infection. This hypothesis was tested by investigating the effect of a high-fiber (HFB) diet on bacterial translocation in rats. Wistar rats were fed a chow or HFB diet for 2 weeks, and then subjected to hepatectomy or to a sham operation. Mesenteric lymphadenectomy or a sham operation were performed in each group. Two weeks later, hepatocytes were isolated, an enteric bacterial suspension was given, and survival of bacteria in mesenteric lymph nodes and liver was examined. Rats fed the HFB diet had higher serum levels of liver-related enzymes. After hepatectomy, the ratio of rats with bacterial translocation was higher in the chow diet group than in the HFB diet group. In the hepatectomy group, survival rates of fecal aerobic and facultative anaerobic bacteria were significantly lower in the chow diet group than in the HFB diet group. Serum levels of cecal alpha- and beta-defensins were higher in the chow diet group than in the HFB diet group. However, the ratio of gram-positive cocci to gram-negative bacilli was higher in the chow diet group. There was no difference in sepsis incidence in the HFB diet group between the bile duct ligation and the sham operation groups. These results suggest that a HFB diet may reduce the bacterial translocation in rats with hepatic failure and may provide an experimental basis for the prevention of bacterial infection in cirrhosis patients.The long-term objective is to
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DX Ball 2 Full Version 776 levels
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DX Ball 2 Full Version 776 levels
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