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. Wind turbine comes in different sizes. It is rated in terms of its capacity in. Tower may be vertically or horizontally mounted or even used in situ. Â .S-type cysteine proteinases of Candida albicans: cloning, sequence analysis and expression of extracellular cysteine proteinase genes.
The gene encoding the major extracellular proteinase of Candida albicans (Cae1p), was subcloned from the genomic DNA libraries of C. albicans. The cloning and sequence analysis of the Cae1p gene revealed that it contains a 3092-bp open reading frame (ORF) that encodes a 997-amino acid protein with a 14-amino-acid-long putative signal sequence. The recombinant Cae1p expressed in Pichia pastoris cells was purified to homogeneity. It showed the same properties as the corresponding mature Cae1p purified from the culture supernatant of C. albicans. The expression of the cae1 gene was highly induced by carbon or nitrogen sources but repressed by sulfur amino acids. The Cae1 proteinase was active against gelatin, collagen, fibrinogen and hemoglobin at low concentrations but had a high specificity for hemoglobin with a Ki value of 0.08 mg/ml. Based on the sequence homologies with the cathepsin S-type cysteine proteinase family, we propose that Cae1p can be classified into the cysteine proteinase family 1, with a molecular weight of 28,340 Da, an isoelectric point of 4.7, and a very high G-X*G-X*G-X*E-G-X*G (cleavage site) activity. The expression and structural characteristics of the cpe1 gene encoding a 28,456-Da protease closely resemble those of the cae1 gene, suggesting that these two genes are products of the same gene and thus both are cysteine proteinases of C. albicans.Q:
Retrieving JSON Data from an HTTP Response
I’ve been trying to retrieve information from JSON data that is returned from an HTTP server. I don’t want a response body and would rather access the JSON data that is returned. I’ve been trying to use the jsonlite package but all it did was provided me with an error.
This is the code I’m using to request the JSON data:
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