Vergiss Es Nie Noten.pdf
Category:18th-century classical composers
Category:18th-century German composers
Category:18th-century male musicians
Category:18th-century keyboardists
Category:18th-century keyboardists
Category:18th-century German people
Category:German classical composers
Category:German classical pianists
Category:German male pianists
Category:German organists
Category:Male organists
Category:People from Nuremberg
Category:1710 births
Category:1779 deaths
Category:German classical composers of church music
Category:German male classical composersGlucose-dependent inhibition of glucose transport in perfused rat hearts.
In perfused rat hearts, the transport of glucose across the myocardial cell membrane is mediated by facilitative hexose carriers. This transport system is inhibited by D-glucose in the presence of sodium in the incubation medium, a phenomenon known as glucose-dependent inhibition. In order to determine if the effect of D-glucose on glucose transport is time-dependent, time-response experiments were performed using hearts that had been perfused with Krebs-Henseleit (KH) buffer containing the substrate and D-glucose. These hearts were prepared in the same manner as hearts used for the time-response studies. Hearts were then transferred to KH buffer without added D-glucose and the time-response of glucose transport to the same glucose concentration was followed by microperfusion experiments. The results indicate that within 10 s after the addition of D-glucose, glucose transport is inhibited in a time-dependent manner. Since previous studies have shown that this inhibition can be prevented by the addition of D-glucose to the perfusate, it was concluded that this inhibition is a result of the binding of the glucose molecule to its carriers. Since these findings are in agreement with other reports in the literature, the ability of the glucose transporter to bind glucose was examined in the present study. Hearts were perfused in the presence of 25 mM glucose and 0.25% bovine serum albumin (BSA). Tritiated D-glucose was then added to the perfusion fluid, and the extent to which the label was bound to the myocardial cell was determined. The data show that the transporter binds glucose with high affinity (Kd = 0.32 +/- 0.12 mM).Democratic Rep. Tulsi Gabbard has also been
, Janz, S3vN
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