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Integrated Microfluidics Devices for Efficient, Specific, and Reproducible Preparation of Biomaterial-Incorporated Gold Nanoparticle Dispersion.
In the present study, we have developed methods for the facile, efficient, and reproducible preparation of biomaterial-incorporated gold nanoparticle (AuNP) dispersion. The approach utilizes integrated microfluidics devices, where the AuNPs are synthesized at the interface between immiscible aqueous and organic phases with the presence of a protein, surfactant, and polymer functionalized on the interface. The synthesized AuNPs are then deposited at the interface of the organic and immiscible aqueous phases where they are preserved, and sustained, under vigorous agitation to form stable and nonaggregated NPs in the aqueous phase. The NPs are subsequently harvested, washed, and characterized by UV-visible spectrophotometry, transmission electron microscopy, and dynamic light scattering. These methods are suitable for high-throughput preparation of large quantities of the biomaterial-incorporated NPs as demonstrated by the preparation of AuNP dispersions, each containing a different biomolecule. The NPs are characterized in terms of stability, size, and morphology, as well as their surface and charge properties. The approach is not limited to only metallic NPs and can be applied to any NPs, irrespective of their size, shape, and morphology. It also
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